Parasite Preparations

Purification Schedule
Parasites are purified on a regular schedule to ensure freshness of the product.   Giardia lamblia cysts are collected and purified daily.   Cryptosporidium parvum oocysts are purified approximately every one to two months.   Our continuous production of these two parasites allows us to provide fresh organisms promptly.  

Microsporidial spores, Giardia muris , and Cryptosporidium muris are purified on a less frequent basis, or as needed.   A lead-time of one to two weeks is sometimes necessary when ordering these three organisms.

Purity
All preparations are guaranteed to be 95% - 99% purified.   Each organism is purified by sucrose and percoll gradients and water washes, as needed.   Each suspension is then viewed microscopically for contamination.

Storage Solution
All organisms can be purchased suspended in a phosphate-buffered saline (PBS) with antibiotics and 0.01% Tween 20, or suspended in 5% formalin with 0.01% Tween 20.   We can also prepare a specific solution, as requested by the customer.

Inactivation
Different methods of inactivating the organisms include freeze-treating and heat-treating.   All information regarding the preparation of the parasite suspension is included on the Product Specification and Analysis Report that accompanies each shipment of parasites.


Giardia lamblia

Catalog Number: P101
Cysts of Giardia lamblia
Human isolate H-3, passaged through gerbils.


»P101 @ 1x10/6 (1 million)

»P101 @ 3x10/6 (3 million)

»P101 @ 5x10/6 (5 million)

»P101 @ 1x10/7 (10 million)

»P101 @ 5x10/7 (50 million)

»P101 @ 1x10/8 (100 million)


Cryptosporidium parvum

Catalog Number: P102C
Oocysts of Cryptosporidium parvum
Iowa isolate (Harley Moon), passaged through calves.

»P102C @ 1x10/6 (1 million)

»P102C @ 5x10/6 (5 million)

»P102C @ 1x10/7 (10 million)

»P102C @ 5x10/7 (50 million)

»P102C @ 1x10/8 (100 million)

»P102C @ 1x10/9 (1000 million)


Catalog Number: P102M
Oocysts of Cryptosporidium parvum
Iowa isolate (Harley Moon), passaged through mice.

»P102M @ 1x10/6 (1 million)

»P102M @ 5x10/6 (5 million)

»P102M @ 1x10/7 (10 million)

»P102M @ 5x10/7 (50 million)

»P102M @ 1x10/8 (100 million)


Encephalitozoon intestinalis

Catalog Number: P103
Spores of Encephalitozoon intestinalis
Didier isolate, from culture with RK-13 cells.

»P103 @ 1x10/6 (1 million)

»P103 @ 5x10/6 (5 million)

»P103 @ 1x10/7 (10 million)

»P103 @ 5x10/7 (50 million)

»P103 @ 1x10/8 (100 million)


•Spores of E. cuniculi and E. hellum are also availabe.


Cryptosporidium muris

Catalog Number: P104
Oocysts of Cryptosporidium muris
RN66 rat isolate from Okasaka, Japan, passaged through mice.


»P104 @ 1x10/6 (1 million)

»P104 @ 5x10/6 (5 million)

»P104 @ 1x10/7 (10 million)

»P104 @ 5x10/7 (50 million)

»P104 @ 1x10/8 (100 million)


Giardia muris

Catalog Number: P105
Cysts of Giardia muris
Roberts-Thomson isolate, passaged through mice.


»P105 @ 5x10/6 (5 million)

»P105 @ 1x10/7 (10 million)

»P105 @ 5x10/7 (50 million)

»P105 @ 1x10/8 (100 million)